E-ISSN:2456-3110

Research Article

Jatipatradi Churna

Journal of Ayurveda and Integrated Medical Sciences

2022 Volume 7 Number 4 May
Publisherwww.maharshicharaka.in

Pharmaceutico Analytical Study of Jatipatradi Churna

Nandennavar V.1*, Kulkarni P.2
DOI: http://dx.doi.org/10.21760/jaims.7.4.4

1* Veena Nandennavar, Assistant Professor, Dept. of Rasashastra and Bhaishajya Kalpana, BVVS Ayurvedic Medical College and Hospital, Bagalkot, Karnataka, India.

2 Pavan Kulkarni, Assistant Professor, Dept. of Rasashastra and Bhaishajya Kalpana, BVVS Ayurvedic Medical College and Hospital, Bagalkot, Karnataka, India.

Oral thrush is an opportunistic infection of the oral cavity. It is common and underdiagnosed among the elders. It is also called as oral candidiasis and is a condition in which the fungus Candida albicans accumulates on the lining of mouth. Candida is a normal organism in our mouth, but sometimes it can overgrow and cause symptoms. The incidence of C Albicans is 45% in neonates, 45-65% in healthy children, 30-45% of healthy adults, 50-65% of people who wear removable dentures, 90% of patients with acute leukaemia and undergoing chemotherapy and 95% of HIV infected patients. Oral thrush causes creamy white lesions, usually on the tongue or inner cheeks. Sometimes oral thrush may spread to the roof of mouth, gums or tonsils, or the back of throat. Jaatipatradi Choorna is one of the formulations explained in Yogaratnakara in Dantaroga Chikitsadhaya containing the ingredients like Jaatipatra, Punarnava, Gajapippali, Kushta, Vacha, Shunti, Ajamoda, Haritaki and Tila which are having Krimighna and Khandughna property. Hence, this present study has been undertaken to prepare Jaatipatradi Choorna and subjecting it to standard analytical parameters of Churna Kalpana.

Keywords: Asanas, Obesity, Sthaulya, Shaileyadi Churna, Udvartana

Corresponding Author How to Cite this Article To Browse
Veena Nandennavar, Assistant Professor, Dept. of Rasashastra and Bhaishajya Kalpana, BVVS Ayurvedic Medical College and Hospital, Bagalkot, Karnataka, India.
Email: 		Veena Nandennavar, Pavan Kulkarni, Pharmaceutico Analytical Study of Jatipatradi Churna. J Ayu Int Med Sci. 2022;7(4):21-26.
Available From
https://jaims.in/jaims/article/view/1835

Manuscript Received Review Round 1 Review Round 2 Review Round 3 Accepted
2022-03-17 2022-03-19 2022-03-26 2022-04-02 2022-04-09
Conflict of Interest Funding Ethical Approval Plagiarism X-checker Note
Nil Nil Yes 19%

© 2022by Veena Nandennavar, Pavan Kulkarniand Published by Maharshi Charaka Ayurveda Organization. This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ unported [CC BY 4.0].

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Nandennavar V et al: Pharmaceutico Analytical Study of Jatipatradi Churna

Introduction

India has a huge heritage of traditional herbal medicine. The Ayurvedic system of medicine has described various herbal formulations in the treatment of diseases, which play an important role in modern health care and curing various ailments. Ayurveda in recent era is attracting global attention due to its holistic approach in the treatment of disease and with minimal adverse drug reaction. The invention of Panchavidha Kashaya Kalpana by Acharya Agnivesha, is really a revolutionary step in Ayurvedic Pharmaceutics.[1] Earlier people used to consume leaves, roots, tubers etc. in a raw form to appease hunger and to treat health problems. Later the invention of Panchavidha Kashaya Kalpana came into existence i.e., Swarasa, Kalka, Kashaya, Hima and Phanta being the fundamental preparation for all other preparation. To bring about the increased shelf life the Upakalpanas came into existence. Churna being the Upakalpana of Kalka Kalpana is defined as the dried fine powder of drug obtained after pounding in Khalwa Yantra (stone mortar) and filtering through cloth is known as Churna.[2] It is also referred as Raja and Kshoda. Jaatipatradi Choorna is one of the formulations explained in Yogaratnakara[3] in Dantaroga Chikitsadhaya containing the ingredients like Jaatipatra, Punarnava, Gajapippali, Kushta, Vacha, Shunti, Ajamoda, Haritaki and Tila which are having Krimighna and Khandughna property. Jaatipatra is attributed to have many medicinal properties. It’s being used in treating the ailments of Mukha Roga and has the Karmukata of Danta Drudata, Vruna Shodhana and Vruna Ropana. Hence, this present study has been undertaken to prepare Jaatipatradi Choorna and subjecting it to standard analytical parameters of Churna Kalpana.

Objectives of the Study

1. To prepare Jaatipatradi Choorna according to classics.

2. Physico-chemical analysis of individual drugs and Jaatipatradi Choorna.

Materials and Methods

  • The raw materials required for the preparation of the Jatipatradi Choorna were collected from reliable sources.
  • Authentication of the ingredients was done from Dravyaguna Department of BVVS Ayurvedic Medical College, Bagalkot.
  • Preparation of Jatipatradi Churna was carried out at the Pharmacy of BVVS Ayurvedic Medical College and Hospital, Bagalkot, Karnataka.
  • The Antimicrobial Activity of the drug was carried out at the Maratha Mandal’s Dental College and Research Institute, Belagavi. Karnataka.

Ingredients of Jatipatradi Churna

Table 1: Showing ingredients of Jatipatradi Churna

SN Ingredient Botanical Name Part used Quantity
1.          Jatipatra Jasminum grandiflorum Linn Leaf 200gm
2.          Punarnava Boerhavia diffusa Root 200gm
3.          Gajapippali Scindapsus officinalis Fruit 200gm
4.          Badara Twak Ziziphus mauritiana Bark 200gm
5.          Kusta Saussurea lappa Rhizome 200gm
6.          Vacha Acorus calamus Linn Root 200gm
7.          Shunti Zingiber officinale Rhizome 200gm
8.          Ajamoda Trachyspermum ammi Fruit 200gm
9.          Haritaki Terminalia chebula Fruit 200gm
10.       Tila Sesamum indicum Seed 200gm

Pharmaceutical Procedure[4]

Each drug was identified, collected and authenticated individually. Each drug was taken separately in the Ulukhala Yantra and pounded to reduce in its particle size and then put in the pulveriser to make Sookshma (fine) Choorna.

The fine Churnas of all the drugs like Jatipatra, Punarnava, Gajapippali, Badara Twak, Kusta, Vacha, Shunti, Ajamoda, Haritaki and Tila were taken in 100gm quantity each in a large stainless-steel vessel. It was then completely mixed.

To attain homogenous mixture, it was put in grinder and collected.

Obtained Jatipatradi Choorna was weighed and then packed in air tight plastic containers.

Analytical methods[5]

1. Organoleptic Characteristics: The colour, odour and texture of Jaatipatradi Choorna was assessed.

2. Physicochemical analysis: Jaatipatradi Choorna was analysed for loss on drying,


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extractive values, ash values, pH as per the standard method prescribed in Ayurvedic Pharmacopeia of India (API).

3. Phytochemical analysis: Jaatipatradi Choorna was analysed for preliminary phytochemical analysis as prescribed in Ayurvedic Pharmacopeia of India (API).

4. HPTLC study: This study was carried out for Jaatipatradi Choorna.

Standard operating procedure of preparation of Churna (Powder)

Collection

↓

Identification and authentication of raw drugs

↓

Drying

↓

Finely pounding in Pulveriser

↓

Finely powdered drug should pass through sieve number 85

↓

Weigh each ingredient separately

↓

Mixing in the specified proportion

↓

Packing in air tight containers

↓

Labeling and

↓

Storage

Table 2: Organoleptic characters of Jatipatradi Churna

SN Jatipatradi Churna Results
1. Form Churna
2. Colour Light brown
3. Taste Sour, pungent & Bitter
4. Odour Characteristic

Table 3: Physicochemical analysis of Jatipatradi Churna.

SN Tests Results
1.          Moisture content 2.385%
2.          Total Ash Value 9.045%
3.          Acid insoluble Ash 2.833%
4.          Water soluble extractive 4.456%
5.          Alcohol soluble extractive 5.594%
6.          pH 6.3

Table 4: Preliminary Phytochemical study of Jatipatradi Churna.

SN Test Aqueous Alcoholic
1.          Test for Carbohydrates Positive Positive
2.          Test for Reducing sugar Positive Positive
3.          Test for Monosaccharide Positive Positive
4.          Test for Pentose sugar Negative Negative
5.          Test for Non Reducing Sugar Negative Negative
6.          Test for Hexose Sugar Negative Positive
7.          Test for Protein Positive Positive
8.          Test for Amino Acids Positive Positive
9.          Test for Steroids Negative Positive
10.       Test for Flavonoids Positive Positive
11.       Test for Alkaloids Positive Positive
12.       Test for Tannins Positive Negative

TLC report

Tests Results
TLC: Alcohol Extract Rf Values
Mobile Phase - Toluene Ethyl acetate Short Wave: 0.07, 0.11, 0.15, 0.57, 0.66, 0.77
Ratio: 7:3 Long Wave: 0.07, 0.11, 0.17, 0.22, 0.25, 0.28, 0.38, 0.45, 0.53, 0.59, 0.67, 0.79, 0.89, 0.92
Tests Results
TLC: Water Extract Rf Values
Mobile Phase-Toluene Ethyl acetate Short Wave: 0.08, 0.11, 0.15, 0.17, 0.23, 0.29, 0.42, 0.63, 0.67, 0.77
Ratio: 7:3 Long Wave: 0.05, 0.10, 0.14, 0.22, 0.28, 0.39, 0.47, 0.52, 0.63, 0.67, 0.78

jaims_1835_01`.JPG



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Nandennavar V et al: Pharmaceutico Analytical Study of Jatipatradi Churna

HPTLC: 1g of Jatipatradi Choorna was suspended in 10ml of alcohol. 3, 6 and 9µl of the above extracts were applied on a pre-coated silica gel F254 on aluminium plates to a band width of 7 mm using Linomat 5 TLC applicator. The plate was developed in Toluene: Ethyl acetate (4.0 : 1.0). The developed plates were visualized under short UV, long UV and then derivatised with vanillin sulphuric acid and scanned under UV 254nm, 366nm and 620nm (After derivatisation). Rf, colour of the spots and densitometric scan were recorded.
jaims_1835_02.JPGFigure 1: TLC photo documentation of Ethanol extract of Jatipatradi Choorna.

track 1 - Ethanol extract of Jatipatradi Choorna – 3 µl
track 2 - Ethanol extract of Jatipatradi Choorna – 6µl
track 3 - Ethanol extract of Jatipatradi Choorna – 9 µl
Solvent system: Toluene: Ethyl acetate (4:1)

Table 1: Rf values of all the sample of Ethanol extract of Jatipatradi Choorna

At Short UV At long UV After derivatisation
- 0.03 (F. blue) -
- 0.05 (F. blue) -
0.12 (D. green) 0.12 (F. blue) -
- 0.15 (F. blue) 0.15 (Purple)
0.22 (D. green) - -
- 0.25 (F. blue) -
- 0.34 (F. red) -
- - 0.37 (Purple)
0.41 (D. green) - -
- - 0.42 (Purple)
- 0.51 (F. red) 0.51 (Purple)
- 0.62 (F. red) -
- 0.73 (F. red) -
- 0.92 (F. red) 0.92 (Purple)

*F-fluorescent

jaims_1835_03.JPGjaims_1835_04.JPGFigure 2. HPTLC photo documentation of Ethanol extract of Jatipatradi Choorna.

At 254nm
jaims_1835_05.JPGjaims_1835_06.JPG

At 366nm
jaims_1835_07.JPG


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jaims_1835_08.JPG

Remarks: The given sample has been analytically standardized as per standard testing protocol. Results of Jatipatradi Choorna samples HPTLC photo documentation, densitometric scan, Rf values are presented in respective tables and figures.

Discussion

On reviewing over the drugs of Jatipatradi Choorna, it is seen to contain Katu and Tikta Rasa Pradhanata. It is Shresta Krimighna, Shodhana, Vedanastapaka, Shothahara, Shoolaprashamana and with its Veerya being Ushna, it is Kapha Vata Shamaka.

Tikta Rasa has Pitta Shamaka property and helps in removing the debris and helps in improving and maintaining the oral hygiene. The Kashaya Rasa helps in reducing the burning sensation.

Physico-chemical parameters of Jatipatradi Choorna were suggestive of the quality of Jatipatradi Choorna and increased shelf life. Moisture content of drug is one of the important factors to determine shelf life. Moisture content of drug is one of the important factors to determine shelf life.

Drugs which are hygroscopic in nature tend to get deteriorated because of high moisture content i.e., higher the moisture content greater the chances of drug to get spoiled.

Phytochemical analysis results showed presence of both primary and secondary metabolites viz. Carbohydrates, reducing sugars, hexose sugars, tannins, phenolic compounds, alkaloids and Saponin glycosides.

Some drugs also showed presence of flavonoids, steroids, anthraquinone, cardiac glycosides, fats and oils. pH of Jatipatradi Choorna was 6.3 (with 10 % aqueous solution) which is a weak basic i.e., Jatipatradi Choorna is mucosa friendly, does not cause harm to the oral mucosa and maintains integrity of oral mucosa.


Conclusion

Thus, the Jatipatradi Choorna was being prepared by following the SOP and was analysed. The analytical parameters were within the parameters mentioned in the API and were suggestive of the genuinely of the raw material used and the quality of the end product obtained.

Reference

1. Agnivesha, Charaka Samhita with Chakrapani teeka, redacted by Kashinath Shastri, Chaukamba Ayurveda Samsthan, reprint edition, 2012. Charaka Sutra Sthana 4/8 Pg no – 57.

2. Sharngadharacharya, Sharngadhara Samhita, Dr. G. Prabhakar Roa. Choukhamba Sanskrit Sansthana, edition 1st 2013 Madhyama Khanda Chapter 6 Shloka no1, Pg no – 178.

3. Vaidya Shastri Lakshmipati’s Yogaratnakar, Edited by Bhisagrantha Brahmasankar Shastri, Chaukhambha Prakashan Varanasi, Reprint 2013, Part 1, Pg no 412.

4. Sharangdhara Samhita by Prof Murthy Srikantha KR. Jaikrishnadas Ayurved Series, Chaukhambha Orientalia, Varanasi. Reprint edition 2012. Madhyama khanda Ch 6, Pg no 84, slokha no 1.

5. Ayurvedic Pharmacopoeia of India Part 1, e-book, Appendix volume(s)- 1,2,3,4, Pg 14, Government of India, Ministry of Health and Family welfare, Department of AYUSH 2007.


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