E-ISSN:2456-3110

Research Article

Ardrakadi Ghrita

Journal of Ayurveda and Integrated Medical Sciences

2022 Volume 7 Number 9 October
Publisherwww.maharshicharaka.in

Experimental evaluation of Ardrakadi Ghritam for its Anti-microbial Activity

Vivek Raj R.1*, Chaitra L.2
DOI: http://dx.doi.org/10.21760/jaims.7.9.11

1* R Vivek Raj, Post Graduate Scholar, Department of Rasa Shastra & Bhaishajya Kalpana, Ramakrishna Ayurvedic Medical College, Yelahanka, Bengaluru, Karnataka, India.

2 LV Chaitra, Associate Professor, Department of Rasa Shastra & Bhaishajya Kalpana, Ramakrishna Ayurvedic Medical College, Yelahanka, Bengaluru, Karnataka, India.

Background: Ardrakadi Ghrita mentioned in Sahasrayogam and its Anti-microbial activity was analyzed. Aim and objective: An experimental evaluation of Ardrakadi Ghrita for its Anti-microbial activity. Methods: Experimental evaluation of Ardrakadi Ghritam for its Anti-microbial activity against microbes such as Escherichia Coli and Klebsiella Pneumonia. Results: Determination of zone inhibition by Agar well diffusion method. For Test Organism are Escherichia Coli and Klebsiella Pneumonia. Inoculum: Cell suspension prepared from cultures grown on Trypsin broth adjusted to 1-2 x105cells/ml. Conclusion: From the experimental study the result it was found that Ardrakadi Ghrita not showed ant- microbial activity.

Keywords: Ardrakadi Ghrita, Anti-microbial activity

Corresponding Author How to Cite this Article To Browse
R Vivek Raj, Post Graduate Scholar, Department of Rasa Shastra & Bhaishajya Kalpana, Ramakrishna Ayurvedic Medical College, Yelahanka, Bengaluru, Karnataka, India.
Email: 		R Vivek Raj, LV Chaitra, Experimental evaluation of Ardrakadi Ghritam for its Anti-microbial Activity. J Ayu Int Med Sci. 2022;7(9):75-77.
Available From
https://www.jaims.in/jaims/article/view/2079

Manuscript Received Review Round 1 Review Round 2 Review Round 3 Accepted
2022-08-29 2022-08-31 2022-09-07 2022-09-14 2022-09-21
Conflict of Interest Funding Ethical Approval Plagiarism X-checker Note
Nil Nil Yes 18%

© 2022by R Vivek Raj, LV Chaitraand Published by Maharshi Charaka Ayurveda Organization. This is an Open Access article licensed under a Creative Commons Attribution 4.0 International License https://creativecommons.org/licenses/by/4.0/ unported [CC BY 4.0].

Journal of Ayurveda and Integrated Medical Sciences 2022;7(9)75
Raj V et al: Experimental evaluation Ardrakadi Ghritam its Anti-microbial Activity

Introduction

Ayurveda is the ancient Indian system of health care and longevity. It involves a holistic view of men his health and illness, Ayurvedic treatment is aimed at patient as an organic whole and treatment consists of salubrious use of drugs, diets and certain practices, currently Ayurveda is widely practiced in the Hindustan peninsula (India and the neighbouring countries) and in recent years, has attracted much attention in economically developed countries such as those in Europe and in the United States and Japan.

This 5000 years old system of medicine recommends treatments with specific herbs and minerals to cure various diseases. Botanicals in the Ayurvedic material medicals have been proven to be safe and effective through several hundred to several thousand years of use. In modern pharmaceutical science, the method of preparation[1] is selected depending upon the Physico-chemical properties of the crude drugs and the main motto of the method adopted for a particular preparations of any drug has to extract the maximum pharmaceutically active ingredients of the crude drug. Therefore, the nature of pharmaceutically active ingredient is an important factor in deciding the method of preparation.

Ardrakadi Ghrita recommended for Kamala contains Ardraka, Haridra, Eranda, Trivrit, Ghrita and Milk. If the dry substance or a liquid is described to be taken in the quantity of Prastha Mana, then in actual practice such substance doubling of quantity is not necessary , Doubling of the quantity of wet and liquid substance is necessary because of their moisture content.[2] If the dry is mentioned in the text to be used in the quantity of Tula, then it should never be doubled even if it is a wet drug.[3] In modern science, percutaneous drainage associated with antibiotic therapy in sever condition even surgery is advised. Medicines may cause side effects, such as itching, nausea, vomiting, seizures etc. Hence the physician is looking for safe and effective medicine without side effects. In the view to contribute a safe and effective, these formulations were taken for the present study.

Materials and Methods

Raw drugs of Ardrakadi Ghritam was procured from Kerala.

Herbal drugs (Ardraka, Haridra, Eranda, Trivrit) are authenticated from Department of Dravya Guna, Ramakrishna Ayurvedic Medical College, Yelahanka, Bangalore. The test drug was prepared from Department of Rasa Shastra & Bhaishajya Kalpana, Ramakrishna Ayurvedic Medical College, Yelahanka, Bengaluru.

In the present study, a sample of Ardrakadi Ghritam was given for laboratory analysis at Skanda life Sciences Pvt. Ltd, Bengaluru.

Determination of zone inhibition by Agar well diffusion method. For Test Organism are Escherichia Coli and Klebsiella Pneumonia. Inoculum: Cell suspension prepared from cultures grown on Trypsin broth adjusted to 1-2 x105 cells/ml.

Table 1: Group Allocation

Test Organisms Test Compounds Conc. Per well
K. pneumoniae Control (Methanol) (20µl) 100%
Ciprofloxacin (Standard) (20µl) 2(µg/ml)
Sample (20µl) 2(mg/ml)
Sample (10µl) 1(mg/ml)
Control (Methanol) (20µl) 100%
E. coli Ciprofloxacin (Standard) (20µl) 2(µg/ml)
Sample (20µl) 2(mg/ml)
Sample (10µl) 1(mg/ml)

Determination of zone inhibition by Agar well diffusion method. For Test Organism are Escherichia Coli and Klebsiella Pneumonia.

Inoculum: Cell suspension prepared from cultures grown on Trypsin broth adjusted to 1-2 x105 cells/ml.

Observations and Results

Table 2: Inhibitory activity of test compounds against test organisms.

Test Organisms Test Compounds Conc. Per well Zone of inhibition (mm) Figure reference number
K.pneumoniae Control (Methanol) (20µl) 100% - Fig 2
Ciprofloxacin (Standard)(20µl) 2(µg/ml) 22
Sample (20µl) 2(mg/ml) -
Sample (10µl) 1(mg/ml) -
Control (Methanol) (20µl) 100% -
E.coli Ciprofloxacin (Standard)(20µl) 2(µg/ml) 26 Fig 3
Sample (20µl) 2(mg/ml) -
Sample (10µl) 1(mg/ml) -

Journal of Ayurveda and Integrated Medical Sciences 2022;7(9)76
Raj V et al: Experimental evaluation Ardrakadi Ghritam its Anti-microbial Activity

The study showed that the Ardrakadi Ghritm was not showing Anti-microbial activity against microbes such as Escherichia Coli and Klebsiella Pneumonia.

Discussion

Ardraka Ghritam is an Ayurvedic medicine, in herbal ghee form. This herbal ghee is made from special formulations used for Kamala, Kumbakamala and also used in treatment of indigestion, chronic diarrhoea. Ardraka means ginger, which is the main ingredient of this medicine. Effects on Tridosa: It calms Vata with Pitta. Side effects: High dose may cause burning sensation in stomach and diarrhoea. Principle: In this herbal ghee, the herbs are infused in the medium of ghee along with herb al water decoction. Then the solid waste herb materials are filtered at, thus this herbal ghee contains oil soluble and water soluble phyto active principles of medicinal herbs. Shunti is a dried form of Ardraka, which has many properties and differs in Guna, Doshagnata and Karma.

Conclusion

On the basis of experimental observations made in this showed that the Ardrakadi Ghritm was not showing Anti-microbial activity against microbes such as Escherichia Coli and Klebsiella Pneumonia.

Reference

1. Tripathi Brahmanand. Sarngadhara Samhita, 1st ed. Varanasi: Chaukhamba Surbharati Prakashan; 2006.p.125.

2. Tripathi Brahmanand Sarngadhara Samhita 1st ed. Varanasi: Chaukhamba Surbharati Prakashan; 2006. p.54.

3. Bramhananda Tripati. Dipika commentary on book of Sharangadhara samhitha ed. 10. prathamodhyaya: 01; verse 33, 34. Varanasi: Chaukambha Surbharathi, 2011:15.


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