Samanga (Rubia cordifolia)^[13], Tejwaha (Zanthoxylum alatum)^[14] along with Trivrita Ghrita^[15] used as a base for the gel formation.

Materials and Methods

All the drugs were procured from the Brahmanath Pharma Pvt. Ltd, a GMP Certified lab Dist. Ahmednagar Maharashtra in crude form and were identified by the same. Pharmacognostical authentication of all the raw drugs was done based on the morphological features, organoleptic characters.

Table 1: Contents of Ayursenso Gel

Process of formulation

All the raw drugs (25gms each) were dried separately in try dryer. The ingredients with the botanical source and parts used are mentioned above. Then dried drugs were disintegrated by the disintegrator separately in aseptic conditions.

After that all the disintegrated drugs were then ground by the pulverizer. Then all the ground drugs were made to pass through the sieve separately. Each powder was made to pass through the 85 number sieves to get a fine powder.

Each Churna was mixed with 5% alcohol and 8 extracts were recovered. These extracts were added with standardized Trivruta Ghrita and Carbopol 934 of 1% concentration (10gms) with the addition of sodium benzoate 6gms.

The mixture was kept for 6-7hrs to allow the dispersion of Carbopol. After with aid of mechanical stirrer, make a homogenous mixture with 1200 rpm to form gel aspect. Triethanolamine added drop by drop with continuous stirring until it gets converted into perfect desired gel and pH. Packaging and labelling of gel within tubes are done in sterilized conditions.

Normal range of pH of standard oral gel is between 5.5-7.0.^[18]

Ayursenso gel pH is 6.98.

Spreadability

Both glass slides of apparatus were cleaned with alcohol and allowed to dry. 1g of gel was placed between the two glass slides. Then 100g of weight was placed on top slide for 5 minutes to compress the sample up to uniform thickness.

Initial weight (100g) was added in the pan. The weight in the pan was increased until the upward slide starts to move over stationary slide.

The time in seconds(s) require to separate the two slides was noted. The triplicate of each sample was taken and time required and distance travelled by slide was noted.^[19]

On following the same procedure spread ability of Ayursenso gel is found 0.2142gm/cm2

Irritancy test

Mark an area of one square cm on the left-hand dorsal surface. The Sample gel was applied to the specified area and time was noted. On following the same procedure no Irritancy, erythema, oedema was observed.^[20]

High performance Thin Layer Chromatography (HPTLC) profile

Initially sample solutions were prepared. Accurately weighed (250 mg) was taken in methanol and was filtered through Whatman I filter paper. The filtrate was further subjected to chromatographic separation.

The Solvent system used was Chloroform: Methanol (9:1 % v/v). 5 μl of sample solution was applied on pre-coated silica gel 60 F 254 TLC plate (E. Merck) of uniform thickness of 0.2 mm and the plate was developed in the solvent system up to a distance of 8 cm.

The plate was visualized under short and long UV radiation and density of the separated spots was recorded using scanner III. The Rf value of all the track at 254 nm, and 366 nm were recorded and presented respectively.

Peak display densitogram of samples under 254 nm, and 366 nm and comparative spectra at various Rf are placed in figure attached in.^[21,22]

given that its base characteristics are consistent with those of a recognized standard. Ayursenso gel pharmacogenetic evaluation highlighted the unique qualities of this remedy. Numerous standardization parameters were used in this examination, including physical characteristics, physiochemical study, organoleptic study, and chromatographic evaluation.

The PH - 6.98, Moisture Content @110°C - nil, Acid Value - 3.16, Iodine Value - 8.3, Saponification Value - 23.7, Refractive Index - 1.472, Spreadability Test -0.2142gm/cm2. The formulation was free from pathogenic microbial contaminations.

Discussion

Comprehensive testing of Ayur Senso Gel was conducted in a GMP-certified laboratory to evaluate and compare their organoleptic and physicochemical properties. The analysis included color, odor, texture, pH, viscosity, spread ability, homogeneity and HPTLC.

Organoleptic Properties

The color of Ayursenso gel was consistent, with both products showing a uniform translucent appearance. Texture analysis revealed a smooth, non-gritty consistency in both formulations. The spread ability was evaluated by manual application, and both gels exhibited comparable ease of spreading and absorption into the skin without leaving residue.

Physicochemical Properties

The pH values of Ayur Senso Gel and Standard oral Gel were within the skin-friendly range (5.5-7.0.), showing no significant difference. Viscosity tests confirmed a similar gel consistency, ensuring ease of application and retention on the skin surface. Homogeneity tests further demonstrated that both products maintained a uniform distribution of active and inactive ingredients, with no signs of phase separation or granule formation over time.

Ayurvedic text Sharangadhar Samhita also affirm this by stating, “Raktashruti Dantpida Shotham Daham Ch Nashyate" for Kusthadi Yoga. From a modern scientific perspective, the chemical constituents of the Ayursenso gel components exhibit anti-inflammatory, antimicrobial, analgesic, antioxidant properties. These properties help address the fundamental pathology of dental hypersensitivity.