Effect of Integrated Approach of Yoga Therapy (IAYT) on DNA damage

Dr. Amrita Ghimire; Dr. Bikash Raj Ghimire; Dr. Samichha Neupane; Prof. Sanjay Pokharel; Dr.Shwetha Kumari Pokharel

doi:10.21760/jaims.v6i01.1179

Dr. Amrita Ghimire PhD Scholar, Nepal Sanskrit University, Dang, Nepal.
Dr. Bikash Raj Ghimire Senior Consultant, National Ayurveda Research and Training Centre, Kathmandu, Nepal,
Dr. Samichha Neupane Consultant, District Ayurveda Health Centre, Kavre, Nepal,
Prof. Sanjay Pokharel Professor, Nepal Sanskrit University, Dang, Nepal,
Dr.Shwetha Kumari Pokharel Asso. Professor, Nepal Sanskrit University, Dang, Nepal.

DOI: https://doi.org/10.21760/jaims.v6i01.1179

Keywords: DNA damage, Electrophoresis, Cometlength, DNA repair, diabetes, stress, IAYT, metabolism, CASP

Abstract

Aim: To assess the effect of IAYT on extent of betterment of DNA damage in diabetic practicing Yoga. Method: Thirty participants with diabetes recruited from Arogyadhama, Holistic Health Care Home in PrashantiKutiram, at Bangalore, were checked for DNA damage before and after 7 days of IAYT intervention. Age range of participants was from 30 to 65 years.Intervention consisted of intensive residential Yoga program comprising of Asana (physical posture), Pranayama, meditation, devotional sessions, diet modification and interactive sessions on philosophical concepts of Yoga. The damage in the genomic DNA of peripheral blood mononuclear cells was assayed by single cell gel electrophoresis method following the previously described protocol of Singh et al. Analysis of the data was done using CASP and excel. Result: After one week of IAYT program, 63% of participants showed lessening in the DNA damage (decrease in post tail length) after intervention while in 37% the DNA damage increased (increase in post tail length). The percentage of decrease of tail length was significantly higher (37%, comparing the percentage of means of pre and post) than the percentage of increase of tail length (19%). The data of both positive and negative change showed normal distribution. Conclusion: DNA Damage has a direct link with defects in metabolism, non-communicable disease like diabetes andstress. There was significant reduction in the tail length of DNA and the total comet length after IAYT intervention which signifies a better improvement in the DNA damage. Whether this was achieved because of reduction in stress or through another physiological pathway is not known. Normal distribution of data shows that the damage or betterment of damage, are both not a chance occurring in this data but first of a kind report of Yoga influencing the DNA repair mechanism. Also, in this novel study we have made findings to differentiate between short and long fragment DNA damage through data analysis.

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