Comparative Phyto-Pharmacognostic study of Field collected and Pharmacy sample of Arjuna (Terminalia arjuna) Bark

Sushma Dongre; Pande Shishir

Sushma Dongre Assistant Professor
Pande Shishir Ph.D. Guide & HOD, Dept. of Rasashashtra and Bhaishjya Kalpana, ASS Ayurved College, Nashik, Maharashtra, India.

Keywords: Arjuna, Terminalia arjuna, Pharmacognostic study, Ayurveda, Physicochemical parameters

Abstract

Arjuna (Terminalia arjuna) is the most widely used drug in Ayurveda due to its potential therapeutic competence. Quality assurance is important issue nowadays due to poor collection and storage practices. So, it needs quality control both for small scale and large-scale preparations of Arjuna formulations. Hence present study is planned to compare both field collected and pharmacy sample of Arjuna bark by analytical testing. Macroscopic, microscopic, physicochemical and high performance thin layer chromatography (HPTLC) by using Arjunic acid as a marker is carried out. Macroscopic and microscopic characteristics observed as per Ayurveda Pharmacopeia (API) except color of field collected bark. In Physicochemical parameters, Ash value was higher in pharmacy sample but within limit whereas percentage of water soluble and alcohol-soluble extractives was high in field collected Arjuna as compare to pharmacy sample. Percentage of extractive values was not as per limits of API. Tannin percentage was three times more in field collected Arjuna bark. In HPTLC study, percentage of Arjunic acid was much higher in pharmacy sample (0.072%) than field collected sample (0.054%). Also, the spots observed were more in this sample. 0.60 was the R_f Value for Arjunic acid and one common unknown spot was noticed in both samples. Present study set preliminary data for percentage of Tannins and quantification of Arjunic acid in Arjuna Bark by HPTLC study which is not found in monograph of Arjuna hence can be used as reference for further study.

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