Antibacterial efficacies of brown sea weed Sargassum Wightii on Periodontal Pathogens: An In-Vitro Microbiological Analysis

Nagraj BK; Koregol AC; Hannahson Puladas; Sulakod K; Patil K; Gore S

doi:10.21760/jaims.7.11.9

Nagraj BK Professor and Head, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.
Koregol AC Professor, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.
Hannahson Puladas Post Graduate Scholar, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.
Sulakod K Post Graduate Scholar, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.
Patil K Post Graduate Scholar, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.
Gore S Post Graduate Scholar, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.

DOI: https://doi.org/10.21760/jaims.7.11.9

Keywords: Sargassum wightii, antimicrobial activity, natural derivative, pharmacological effect, periodontal pathogens.

Abstract

Objective: Sargassum wightii is brown algae available across the south east coast of India which can produce many secondary metabolites and are opulent sources of potential plant derived by-products with antiviral, antihelminthic, antifungal and antibacterial activities. The objective of the current study was to assess minimal inhibitory concentration MIC and minimum bactericidal concentration MBC of whole leaf extract of Sargassum wightii on P.Gingivalis (Pg), F.Nucleatum (Fn), T.Forsythia (Tf) and A. Actinomycetemcomitans (Aa). Methods: The method executed out by serial dilutions of the test drug with thioglycollate broth nine times. Cultures of chosen pathogens were added to obtain culture suspension up to 10^-9. Their turbidity has monitored to assess MIC after incubating in oxygen independent container. The First 3 or 5 dilutions tubes which were MIC sensitive were removed and incubated one complete day. To determine whether there was bacterio-static or bactericidal effect of the extract on selected organisms, colony numbers were assessed the day after. Results: Outcome for MIC was in the span of 0.2μg/ml to 0.4μg/ml and for MBC from 0.2μg/ml to 3.12μg/ml. Conclusion: Positive pharmacological effect does exists against tested microorganisms of whole leaf extract of Sargassum Wighitti & demands more extensive research for treating multi-factorial periodontal disease.

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Author Biography

Nagraj BK, Professor and Head, Dept. of Periodontics, PMNM Dental College and Hospital, Bagalkot, Karnataka, India.

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