Study of the Antimicrobial Activity and Synergistic Inhibitory Activity of Carica papaya leaves extract with Antibiotics on Escherichia coli

Shriya S Phadnis; Krupa A Purandare; Kalpana Dhuri

doi:10.21760/jaims.8.8.5

Shriya S Phadnis Post Graduate Scholar, Department of Microbiology, G.N. Khalsa College Autonomous, Matunga, Maharashtra, India.
Krupa A Purandare Assistant Prof., Department of Microbiology, G.N. Khalsa College Autonomous, Matunga, Maharashtra, India.
Kalpana Dhuri Professor & HOD, Dept. of Ayurvedic Paediatrics, D Y Patil School of Ayurveda, Nerul, Navi Mumbai, Maharashtra, India.

DOI: https://doi.org/10.21760/jaims.8.8.5

Keywords: Antimicrobial activity, Agar strip diffusion method, Synergistic, Diarrhoeal treatment, Coliforms, Carica papaya, Escherichia coli, Ayurveda

Abstract

Street food is one of the major causes of diarrhoea and gastrointestinal infections. Escherichia coli are one of the major contributors for many gastrointestinal infections. Natural sources are used to derive drugs that play striking role in the prevention and treatment of human diseases. Ayurveda has described Earandakarkati (Carica papaya) in many digestive disorders. This study was carried out to show the antimicrobial activity of Erandakarkati (Carica papaya) leaves extract alone and in combination with the antibiotics including Penicillin, Erythromycin, Piperacillin and Norfloxacin using agar strip diffusion method to see whether they produce synergistic or an antagonistic effect against street food isolated Escherichia coli and diarrhoeal ETEC strain (as a control). Carica papaya leaves extract showed antibacterial effect. The only combination of papaya leaf extract with Piperacillin resulted into a synergistic effect while the other combinations exhibited an antagonistic effect.

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References

Chumber SK, Kaushik K, Savy S. Bacteriological analysis of street foods in Pune. Indian J Public Health. 2007 AprJun;51(2):114-6. PMID: 18240473.

Foods, F. S. Report of an FAO technical meeting on street foods, Calcutta, 6-9 November 1995. FAO Food and Nutrition paper, 63.

Sheth, M., Gurudasani, R., & Mudbidri, R. (2005). Screening for pathogenic microorganisms in street-vended bhelpuri in urban Vadodara: a HACCP approach. Journal of Food Science and Technology (Mysore), 42(5), 395-399.

Estrada-Garcia T, Lopez-Saucedo C, Zamarripa-Ayala B, Thompson MR, Gutierrez-Cogco L, Mancera-Martinez A, Escobar-Gutierrez A. Prevalence of Escherichia coli and Salmonella spp. In street-vended food of open markets (tianguis) and general hygienic and trading practices in Mexico City. Epidemiol Infect. 2004 Dec;132(6):1181-4. Doi:

10.1017/s0950268804003036. PMID: 15635978; PMCID: PMC2870212.

Das M, Rath CC, Mohapatra UB. Bacteriology of a most popular street food (Panipuri) and inhibitory effect of essential oils on bacterial growth. J Food Sci Technol. 2012 Oct;49(5):564-71. Doi: 10.1007/s13197-010-0202-2. Epub 2011 Jan 12. PMID: 24082267; PMCID: PMC3550854.

Vaidya Bipinlal. Nighantu Adarsh. Chaukhamba Bharati Academy, Varanasi. Second ed, 1998, vol 1 pg 613.

Charan J, Saxena D, Goyal JP, Yasobant S. Efficacy and safety of Carica papaya leaf extract in the dengue: A systematic review and meta-analysis. Int J Appl Basic Med Res. 2016 Oct-Dec; 6(4):249-254. Doi: 10.4103/2229-516X.192596. PMID: 27857891; PMCID: PMC5108100.

Vaidya Bipinlal. Nighantu Adarsh. Chaukhamba Bharati Academy, Varanasi. second ed 1998 vol 1 pg 615.

Jadhav, K., Ahir, K., Desai, S., Desai, S., & Acharya, S. (2022). Isolation and characterization of carpaine and dihydroxy derivative of carpaine from leaves of Carica papaya: Development of fast HPLC method and standardization of formulations. Journal of Chromatography B, 1213, 123533.

Devi, S., Ropiqa, M., Murti, Y. B., & Nugroho, A. K. (2020). Screening of Extraction Process and The Estimation of Total Alkaloids in Carica papaya Linn. Leaf. Majalah Obat Tradisional, 25(2), 90-95.

Evans DJ Jr, Evans DG. Escherichia Coli in Diarrheal Disease. In: Baron S, editor. Medical Microbiology. 4th ed. Galveston (TX): University of Texas Medical Branch at Galveston; 1996. Chapter 25. PMID: 21413261.

Kornacki JL, Marth EH. Foodborne Illness Caused by Escherichia coli: A Review. J Food Prot. 1982 Sep;45(11):10511067. Doi: 10.4315/0362-028X-45.11.1051. PMID: 30913622.

Yadav, N. P., Yadav, R. K., & Prakash, S. (2019). Bacterial Contamination of Street Vended Food Pani Puri Available in Janakpurdham, Dhanusha. Tribhuvan University Journal of Microbiology, 6, 70- 75.

Acharya Vagbha, Ashtang Hridayam Sarvangsundara commentary by Arundatta ed by Paradkar BH. Krishnadas Academy, Varanasi. 1982, Nidaansthan 12/1 pg 513.

Gogate V M Ayurvedic pharmacology and Therapeutic Uses of medicinal plants publ Bhavans SPARC 2000 pg 554-555

Chen L, Deng H, Cui H, Fang J, Zuo Z, Deng J, Li Y, Wang X, Zhao L. Inflammatory responses and inflammation associated diseases in organs. Oncotarget. 2017 Dec 14;9(6):7204-7218. Doi: 10.18632/oncotarget.23208. PMID: 29467962; PMCID: PMC5805548.

Owoyele BV, Adebukola OM, Fummilayo AA, Soladoye AO. Anti-inflammatory activities of ethanolic extract of Caricapapaya leaves. Inflammopharmacology 2008; 16(4): 168-173

Unaeze, Bright C., et al. “Antimicrobial activities of Carica papaya leaf against diarrhoea causing agents.” International Journal of Advanced Engineering Research and Science, vol. 5, no. 8, Aug. 2018.

Attah, F., Moses, E., Abalaka, Shadrach, A.U., & Tayo, O. (2021). In-vitro Investigation on the Therapeutic Potential of Carica papaya Leaf Extract on Some Pathogenic Bacteria.

A. E. Ajiboye and R. A. Olawoyin Antibacterial Activities and Phytochemical Screening of Crude Extract of Carica Papaya Leaf Against selected Pathogens. Global Journal of Pure and Applied Sciences Vol. 26, 2020: 165-170

Alorkpa, E. J., Boadi, N. O., Badu, M., & Saah, S. A. (2016). Phytochemical screening, antimicrobial and antioxidant properties of assorted Carioca papaya leaves in Ghana. Journal of Medicinal Plants Studies, 4(6), 193-198.

Daneman N, Fridman D, Johnstone J, Langford BJ, Lee SM, MacFadden DM, Mponponsuo K, Patel SN, Schwartz KL, Brown KA. Antimicrobial resistance and mortality following E. coli bacteremia. EClinicalMedicine. 2022 Dec 26;56:101781. Doi: 10.1016/j.eclinm.2022.101781. PMID: 36618891; PMCID: PMC9813674.

Adwan, G., & Mhanna, M. (2009). Synergistic effects of plant extracts and antibiotics on Staphylococcus aureus strains isolated from clinical specimens. Asian Pacific Journal of Tropical Medicine, 2(3), 46-51.

Rakholiya, K., & Chanda, S. (2012). In vitro interaction of certain antimicrobial agents in combination with plant extracts against some pathogenic bacterial strains. Asian Pacific Journal of Tropical Biomedicine, 2(3), S1466-S1470.