Phytochemical investigation and experimental evaluation of Panchavalkala for Lekhana Karma w.s.r. to Antihyperlipedemic Activity

Raghunath G.V; M.S Veena; Suresh Janadri

doi:10.21760/jaims.9.2.9

Raghunath G.V Professor & HOD, Dept. of Dravyaguna, Atreya Ayurvedic Medical College, Hospital & Research Centre, Doddaballapura, Bangalore, Karnataka, India.
M.S Veena Professor & HOD, Dept. of PG & PhD studies in Dravyaguna, Govt. Ayurveda Medical College, Bangalore, Karnataka, India.
Suresh Janadri Assistant Professor, Dept. of Pharmacology, Acharya & B. M. Reddy College of Pharmacy, Bangalore, Karnataka, India.

DOI: https://doi.org/10.21760/jaims.9.2.9

Keywords: HFD- High fat diet, PVK-Panchavalkala, Atorvastatin, Hyperlipidemia, AE-Aqueous extract, EE- Ethanolic extract, Anti hyperlipidemic

Abstract

Panchavalkala the barks of five trees i.e., Nyagrodha (Ficus benghalensis L.), Udumbara (Ficus racemosa L.), Ashwatha (Ficus religiosa L.), Plaksha (Ficus virens Aiton) and Parisha (Thespesia populnea (L.)Sol.ex Correa) are also known as Pancha Ksheeri Vrikshas in use since Vedic period. The plant samples were collected in Sharad Rutu (late autumn) from their natural habitat, extracted using ethanol and water and subjected for the phytochemical analysis. The study was aimed to evaluate Antihyperlipidemic effect (Lekhana Karma) of Panchavalkala Bark extracts individually and in combination (1:1:1:1:1) both in ethanolic and aqueous extracts on serum lipid profile in Cafeteria died (HFD) induced hyperlipidemia in SD rats. The animals were divided into fifteen groups of six animals each. The normal control group continued to be fed laboratory pellet chow ad libitum. The cafeteria diet-control group received the cafeteria diet in addition to the normal pellet diet (NPD). The remaining 13 groups were fed with the cafeteria diet and NPD along with standard control Atorvastatin 10mg/kg and the test drug p.o for 30 days. Treatment with the test substance PVK AE and PVK EE at doses of 200mg/kg body weight p.o. controlled and regulated the HFD induced weight gain, glucose and lipids in the blood when compared against high fat diet control (P< 0.05). PVK bark extracts are capable of exhibiting significant antihyperlipidemic activity in HFD induced SD rats. Taken together, this study strongly suggests that PVK bark extracts might be an efficient way for treatment of hyperlipidemia.

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References

Ansarullah, Jadeja RN, Thounaojam MC, Patel V, Devkar RV, Ramachandran AV. Antihyperlipidemic potential of a polyherbal preparation on triton WR 1339 (Tyloxapol) induced hyperlipidemia: A comparison with lovastatin. Int J Green Pharm. 2009; 3:119–24. [Google Scholar]

R. M. Krauss and Y. A. Kesaniemi, “Cardiovascular disease and hyperlipidaemia,” Current Opinion in Lipidology, vol. 5, no. 4, pp. 249–251, 1994.

N. J. Stone, “Lipid management: current diet and drug treatment options,” The American Journal of Medicine, vol. 101, no. 4, supplement 1, pp. 40S–49S, 1996.

Y.Lin, S. S. Mousa, N. Elshourbagy, and S. A. Mousa, “Current status and future directions in lipid management: emphasizing low-density lipoproteins, high-density lipoproteins, and triglycerides as targets for therapy,” Vascular Health and Risk Management, vol. 6, no. 1, pp. 73–85, 2010

Brown MS, Goldstein JL. Multivalent feedback regulation of HMG CoA reductase, a control mechanism coordinating isoprenoid synthesis and cell growth. J Lipid Res. 1980; 21:505–17.

Carbonell T, Freire E. Binding thermodynamics of statins to HMG-CoA reductase. Biochemistry. 2005; 44:11741–8.

Corsini A, Maggi FM, Catapano AL. Pharmacology of competitive inhibitors of HMG-CoA reductase. Pharmacol Res. 1995; 31:9–27.

Baigent C, Keech A, Kearney PM, Blackwell L, Buck G, Pollicino C, et al. Efficacy and safety of cholesterol-lowering treatment: prospective meta-analysis of data from 90,056 participants in 14 randomized trials of statins. Lancet. 2005; 366(9493):1267–78.

Jones PH, Bays HE, Davidson MH, Kelly MT, Buttler SM, Setze CM, et al. Evaluation of a new formulation of fenofibric acid, ABT-335, co-administered with statins: study design and rationale of a phase III clinical programme. Clin Drug Investig. 2008; 28:625–34.

Alsheikh-Ali AA, Karas RH. Adverse events with concomitant amiodarone and statin therapy. PrevCardiol. 2005; 8:95–7.

Joy TR, Hegele RA. Narrative review: statin-related myopathy. Ann Intern Med. 2009; 150(12):858–68.

A role of Ficus species in the management of diabetes mellitus: A review Journal of Ethnopharmacology, Volume 215, 6 April 2018, Pages 210-232

The Ayurveda Pharmacopeia of India Part-I, Vol-V, Ist edition Jan-2006, ISBN:81-901151-6-2.,3-8 printed by NISCAIR, CSIR, Dr. K.S.Krishnan Marg, New Delhi, 110012 and Part-I, Vol-I, ISBN 81-901151-3-8, 1990, 2001

Phytochemical standardization of panchavalkala: An ayurvedic formulation and evaluation of its anticancer activity in cervical cancer cell lines ShamaAphale, Savita Pandita, Prerna Raina, JN Mishra, RuchikaKaul-Ghanekar

Pharmacog.Net,Year :2018, Volume :14 , Issue :58, Page :554-560

NeerajTandan, Review on Indian Medicinal Plants, Vol-II, Published by ICMR, New Delhi – 2013, ISSN:0972-7957, P.B.No.4911, New Delhi – 110029, India

Quality Standards of Indian Medicinal Plants Vol-3, 7, 13, 16, Published by ICMR, V. RamalingaswamiBhawan, Ansari Nagar, P.B.No.4911, New Delhi-110029, India

Sarma, D. S. K., &Babu, A. V. S. (2011). Pharmacognostic and phytochemical studies of Thespesiapopulnea Linn. Journal of Chemical and Pharmaceutical Research, 3(4), 237-244.

Roopashree TS, Dang R, Rani SRH, Narendra C. Antibacterial activity of anti-psoriatic herbs: Cassia tora, Momordicacharantia and Calendula officinalis. International Journal of Applied Research in Natural Products 2008; 1(3): 20-28.

Obasi NL, Egbuonu ACC, Ukoha PO, Ejikeme PM. Comparative phytochemical and antimicrobial screening of some solvent extracts of Samaneasaman pods. African journal of pure and applied chemistry 2010; 4(9): 206-212.

Felipe H.A. Fernandes et.al/ RevistaBrasileira de Farmacognosia, Volume 25, Issue 3, May–June 2015, Pages 208-211

RevistaBrasileira de Farmacognosia Volume 25, Issue 3, May–June 2015, Pages 208-211 Development of a rapid and simple HPLC-UV method for determination of gallic acid in Schinopsisbrasiliensis

Kumar S, Alagawadi KR, Rao MR. Effect of Argyreiaspeciosa root extract on cafeteria diet-induced obesity in rats. Indian J Pharmacol 2011; 43:163-7.

Allian CC, Poon LS, Chan CS, Richmond W, Paul CF. Enzymatic determination of total serum cholesterol. Clin Chem. 1974; 20: 470–5. [PubMed] [Google Scholar]

Pari L, Latha M. Effect of Cassia auriculata flowers on blood sugar levels, serum and tissue lipids in streptozotocin diabetic rats. Singapore Med J. 2002; 43: 617–21.

Dobiasova M, Frohlich J. The Plasma parameter log (TG/HDL-C) as an atherogenic index: correlation with lipoprotein particle size and esterification rate in apoB-lipoprotein-depleted plasma (FER (HDL)). ClinBiochem. 2001; 34:583-8.

Saravana K, Mazumder A, Saravanan VS. Antihyperlipidemic activity of Camellia sinensis leaves in Triton WR-1339 induced albino rats. Pharmacogn Mag. 2008; 4: 60–4. [Google Scholar]

SupravaSahoo et al. /Asian Pac J Trop Biomed 2013; 3(11): 871-876 875

Anandhi R, Thomas PA, Geraldine P. Evaluation of the antiatherogenic potential of chrysin in Wistar rats. Mol Cell Biochem 2014; 385:103–13.

Pandya N, Santani D, Jain S. Antioxidant activity of ezetimibe in hypercholesterolemic rats. Indian J Pharmacol 2006; 38:205–6.

Verlecar XN, Jena KB, Chainy GB. Biochemical markers of oxidative stress in Pernaviridis exposed to mercury and temperature. ChemBiol Interact 2007; 167:219–26.

Sampey BP, Vanhoose AM, Winfield HM, Freemerman AJ, Muehlbauer MJ, Fueger PT, et al. Cafeteria diet is a robust model of human metabolic syndrome with liver and adipose inflammation: Comparison to high-fat diet. Obesity (Silver Spring) 2011; 19:1109-17.

Sclafani A, Springer D. Dietary obesity in adult rats: Similarities to hypothalamic and human obesity syndromes. PhysiolBehav 1976; 17:461-71.

Raghunath G.V.,Veena M.S., Lalitha B.R. Phytochemical investigation and in vitro antioxidant activity of Panchavalkala bark extracts IJAPR Feb-2023/Vol-11/Issue-2/ Page no. 1-12

Raghunath G.V.,Veena M.S., Lalitha B.R. In Vitro Pancreatic lipase inhibition potential of Panchavalkala Aqueous and Ethanolic extracts JAHM 2022; 10(6): 8-24

Visavadiya NP, Narasimhacharya AVRL. Asparagus root regulates cholesterol metabolism and improves antioxidant status in hypercholesteremic rats. Advance Access Publication. 2009; 6:219–26.

Da Silva VB, Taft CA, Silva CHTP. Use of virtual screening, flexible docking, and molecular interaction fields to design novel HMG-CoA reductase inhibitors for the treatment of hypercholesterolemia. J PhysChem A. 2008; 112: 2007–11.

Bioactivity guided fractionation and hypolipidemic property of a novel HMG-CoA reductase inhibitor from FicusvirensAit Danish Iqbal1 , M Salman Khan1*, MohdSajid Khan1 , Saheem Ahmad1 , MdSarfaraj Hussain2 and Mohd Ali3 Iqbal et al. Lipids in Health and Disease (2015) 14:15 DOI 10.1186/s12944-015-0013-6

Khanna AK, Rizvi F, Chander R. Lipid lowering activity of Phyllanthusniruri in hyperlipidemic rats. J Ethnopharmacol 2002; 82:19–22.

Fungwe TV, Cagen LM, Cook GA, Wilcox HG, Heimberg M. Dietary cholesterol stimulates hepatic biosynthesis of triglyceride and reduces oxidation of fatty acids in the rat. J Lipid Res 1993;34:933-41

Buhman KK, Chen HC, Farese RV Jr. The enzymes of neutral lipid synthesis. J BiolChe 2001; 276: 40369-72.

Horton JD, Goldstein JL, Brown MS. SREBPs: Activators of the complete program of cholesterol and fatty acid synthesis in the liver. J Clin Invest 2002; 109:1125-31.

Altmann SW, Davis HR, Zhu LJ. Niemann Pick C1 like 1 protein is critical for intestinal cholesterol absorption. Science 2004; 303:1201-4.

Bioactivity guided fractionation and hypolipidemic property of a novel HMG-CoA reductase inhibitor from FicusvirensAit Iqbal et al. Lipids in Health and Disease (2015) 14:15 DOI 10.1186/s12944-015-0013-6

Assmann G, Nofer JR. Atheroprotective effects of high-density lipoproteins. Annu Rev Med 2003; 54:321-41.

Vergès B. Pathophysiology of diabetic dyslipidaemia: where are we? Diabetologia. 2015; 58:886–899.

Journal of Cardiovascular Pharmacology: Volume 16 - Issue - p S1-S7, Abbate, Samuel L.; Brunzell, John D. Pathophysiology of Hyperlipidemia in Diabetes Mellitus

Journal of Ethnopharmacology, Volume 215, 6 April 2018, Pages 210-232, A role of Ficus species in the management of diabetes mellitus: A review

Shahidi F, Janitha PK, Wanasundara PD. Phenolic antioxidants. Ctit Rev Food SciNutr. 1992; 202:307

Gotto AM, Assmann G, Carmena R, et al. The ILIB lipid handbook for clinical practice: blood lipids and coronary heart disease. 2nd ed. New York, NY: International Lipid Information Bureau; 2000. p. 52.p. 53.p. 201. [Google Scholar]

Genest J, Frohlich J, Fodor G, McPherson R the Working Group on Hypercholesterolemia and Other Dyslipidemias. Recommendations for the management of dyslipidemia and the prevention of cardiovascular disease:

www.ncbi.nlm.nih.gov/pmc/articles/PMC6079547/ Myat Su Bo, WhyeLianCheah, SoeLwin, Tin Moe Nwe, Than Than Win, Myint Aung; Publish Year: 2018

Vasc Health Risk Manag. 2009; 5: 757–765. Published online 2009 Sep 18. PMCID: PMC2747394 PMID: 19774217