Phyto-chemical Standardization of Herbal Formulation (PMM3) for Blood Sugar Attenuating Actions in Streptozotocin induced Rats

Dr. Tapas Kumar Sur; Prabir Kr. Sinha Mahapatra; Alok K. Hazra; Subhas C. Mandal

doi:10.21760/jaims.v2i04.235

Dr. Tapas Kumar Sur Post Doctoral Research Scholar, Department of Pharmacology, Institute of Post Graduate Medical Education & Research 244B, AJC Bose Road- 700 020, West Bengal, India.
Prabir Kr. Sinha Mahapatra Associate Professor, Dept. of Pharmaceutical Chemistry, Institute of Pharmacy & Technology, Salipur, Orissa, INDIA.
Alok K. Hazra Research Officer, Chemistry Division, RKMA Quality Testing Laboratories, Kolkata, West Bengal,
Subhas C. Mandal Professor, Division of Pharmacognosy, Dept of Pharmaceutical Technology, Jadavpur University, Kolkata, West Bengal, India.

DOI: https://doi.org/10.21760/jaims.v2i04.235

Keywords: Diabetes, Herbs, Streptozotocin, HPTLC, Quercetin

Abstract

The present study was intended to prepare herbal formulation, PMM3 using purified and modified parts of five common Indian herbs like, Trigonella foenum-graccum, Tinospora cordifolia, Scoparia dulcis, Adhatoda vasica and Cassia occidental. PMM3 was standardized using physico-chemical, phytochemical, UV-VIS spectral, HPTLC, AAS and GC methods. The phenolics and flavonoids contents were assessed. Anti-hyperglycaemic activities of PMM3 was evaluated on Streptozotocin induced (50mg/kg, i.v) diabetic rats. PMM3 (50-150 mg/kg, p.o) exhibited best potentiality in reducing blood glucose within 14 days treatment in comparison with Diabecon® (Himalaya, India) at the same dose. The preset observation identified formulation PMM3 for anti-hyperglycaemic effect.

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